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lin28 47902 genes  (Addgene inc)


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    Addgene inc lin28 47902 genes
    a Schematic of the process for selecting a key transcription factor to promote stemness of AF-MSCs. b Morphological changes and c growth rate of AF-MSCs infected with retroviral vectors encoding Oct4, Nanog, and <t>Lin28,</t> alone or in combination. Scale bar, 1 mm. d Expression of transcription factors, alone or in combination, as determined by western blotting. e Overexpression of exogenous Nanog in AF-MSCs, detected by reverse transcriptase–polymerase chain reaction (RT-PCR) and immunofluorescence. Scale bar, 1 mm. f Nanog expression in H1 embryonic stem cells, AF-MSCs overexpressing Nanog (AF-N-MSCs), and AF-MSCs. g Expression of Nanog in AF-N (infected at 10 passages) over 40 passages. h Expression of endogenous Nanog in AF-N-MSCs, detected by quantitative real-time RT-PCR. Data are represented as the mean ± SD ( n = 3). *** p < 0.001
    Lin28 47902 Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lin28 47902 genes/product/Addgene inc
    Average 92 stars, based on 6 article reviews
    lin28 47902 genes - by Bioz Stars, 2026-02
    92/100 stars

    Images

    1) Product Images from "Overexpression of Nanog in amniotic fluid–derived mesenchymal stem cells accelerates dermal papilla cell activity and promotes hair follicle regeneration"

    Article Title: Overexpression of Nanog in amniotic fluid–derived mesenchymal stem cells accelerates dermal papilla cell activity and promotes hair follicle regeneration

    Journal: Experimental & Molecular Medicine

    doi: 10.1038/s12276-019-0266-7

    a Schematic of the process for selecting a key transcription factor to promote stemness of AF-MSCs. b Morphological changes and c growth rate of AF-MSCs infected with retroviral vectors encoding Oct4, Nanog, and Lin28, alone or in combination. Scale bar, 1 mm. d Expression of transcription factors, alone or in combination, as determined by western blotting. e Overexpression of exogenous Nanog in AF-MSCs, detected by reverse transcriptase–polymerase chain reaction (RT-PCR) and immunofluorescence. Scale bar, 1 mm. f Nanog expression in H1 embryonic stem cells, AF-MSCs overexpressing Nanog (AF-N-MSCs), and AF-MSCs. g Expression of Nanog in AF-N (infected at 10 passages) over 40 passages. h Expression of endogenous Nanog in AF-N-MSCs, detected by quantitative real-time RT-PCR. Data are represented as the mean ± SD ( n = 3). *** p < 0.001
    Figure Legend Snippet: a Schematic of the process for selecting a key transcription factor to promote stemness of AF-MSCs. b Morphological changes and c growth rate of AF-MSCs infected with retroviral vectors encoding Oct4, Nanog, and Lin28, alone or in combination. Scale bar, 1 mm. d Expression of transcription factors, alone or in combination, as determined by western blotting. e Overexpression of exogenous Nanog in AF-MSCs, detected by reverse transcriptase–polymerase chain reaction (RT-PCR) and immunofluorescence. Scale bar, 1 mm. f Nanog expression in H1 embryonic stem cells, AF-MSCs overexpressing Nanog (AF-N-MSCs), and AF-MSCs. g Expression of Nanog in AF-N (infected at 10 passages) over 40 passages. h Expression of endogenous Nanog in AF-N-MSCs, detected by quantitative real-time RT-PCR. Data are represented as the mean ± SD ( n = 3). *** p < 0.001

    Techniques Used: Infection, Retroviral, Expressing, Western Blot, Over Expression, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Quantitative RT-PCR



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    lin28 47902 genes  (Addgene inc)
    92
    Addgene inc lin28 47902 genes
    a Schematic of the process for selecting a key transcription factor to promote stemness of AF-MSCs. b Morphological changes and c growth rate of AF-MSCs infected with retroviral vectors encoding Oct4, Nanog, and <t>Lin28,</t> alone or in combination. Scale bar, 1 mm. d Expression of transcription factors, alone or in combination, as determined by western blotting. e Overexpression of exogenous Nanog in AF-MSCs, detected by reverse transcriptase–polymerase chain reaction (RT-PCR) and immunofluorescence. Scale bar, 1 mm. f Nanog expression in H1 embryonic stem cells, AF-MSCs overexpressing Nanog (AF-N-MSCs), and AF-MSCs. g Expression of Nanog in AF-N (infected at 10 passages) over 40 passages. h Expression of endogenous Nanog in AF-N-MSCs, detected by quantitative real-time RT-PCR. Data are represented as the mean ± SD ( n = 3). *** p < 0.001
    Lin28 47902 Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lin28 47902 genes/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    lin28 47902 genes - by Bioz Stars, 2026-02
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    a Schematic of the process for selecting a key transcription factor to promote stemness of AF-MSCs. b Morphological changes and c growth rate of AF-MSCs infected with retroviral vectors encoding Oct4, Nanog, and Lin28, alone or in combination. Scale bar, 1 mm. d Expression of transcription factors, alone or in combination, as determined by western blotting. e Overexpression of exogenous Nanog in AF-MSCs, detected by reverse transcriptase–polymerase chain reaction (RT-PCR) and immunofluorescence. Scale bar, 1 mm. f Nanog expression in H1 embryonic stem cells, AF-MSCs overexpressing Nanog (AF-N-MSCs), and AF-MSCs. g Expression of Nanog in AF-N (infected at 10 passages) over 40 passages. h Expression of endogenous Nanog in AF-N-MSCs, detected by quantitative real-time RT-PCR. Data are represented as the mean ± SD ( n = 3). *** p < 0.001

    Journal: Experimental & Molecular Medicine

    Article Title: Overexpression of Nanog in amniotic fluid–derived mesenchymal stem cells accelerates dermal papilla cell activity and promotes hair follicle regeneration

    doi: 10.1038/s12276-019-0266-7

    Figure Lengend Snippet: a Schematic of the process for selecting a key transcription factor to promote stemness of AF-MSCs. b Morphological changes and c growth rate of AF-MSCs infected with retroviral vectors encoding Oct4, Nanog, and Lin28, alone or in combination. Scale bar, 1 mm. d Expression of transcription factors, alone or in combination, as determined by western blotting. e Overexpression of exogenous Nanog in AF-MSCs, detected by reverse transcriptase–polymerase chain reaction (RT-PCR) and immunofluorescence. Scale bar, 1 mm. f Nanog expression in H1 embryonic stem cells, AF-MSCs overexpressing Nanog (AF-N-MSCs), and AF-MSCs. g Expression of Nanog in AF-N (infected at 10 passages) over 40 passages. h Expression of endogenous Nanog in AF-N-MSCs, detected by quantitative real-time RT-PCR. Data are represented as the mean ± SD ( n = 3). *** p < 0.001

    Article Snippet: The amplified fragments were cloned into the NotI-digested pMXs vector (Cell Biolabs). pMXs vectors containing Oct4 (#17217) and Lin28 (#47902) genes were purchased from Addgene (Watertown, MA, USA).

    Techniques: Infection, Retroviral, Expressing, Western Blot, Over Expression, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Quantitative RT-PCR